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Research Laboratory Technician job in Moss Landing

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California (CA), Moss Landing
Employer:  SJSU Research Foundation
Job Ref:  MLML RLT
Country:  United States
City:  Moss Landing
State:  California (CA)
Zip Code:  95039

Position:                                 RESEARCH LABORATORY TECHNICIAN

DEPARTMENT:                        Moss Landing Marine Laboratories

IMMEDIATE SUPERVISOR:    Program Director, Diatom Molecular Genetics Project

POSTING DATE:                     June 19, 2017

CLOSING DATE:                     Posted Until Filled

SALARY:                                  $19.00 to $22.00, DOQ/E
                                                 Full Time

EXEMPT STATUS:                  Non-Exempt (hourly)


GENERAL NATURE OF POSITION:   The Research Laboratory Technician will support research staff in our on-going diatom molecular genetics project team. Specifically, he or she will support projects investigating diatom molecular genetics and physiology of secondary metabolites



1. Maintain and schedule phytoplankton stock cultures and grow outs for electrotransformation experiments. Add to Pseudo-nitzschia isolate collections when present in field samples.

2. Screening and maintenance of transformed cell lines, optimizing grow out and transformation conditions.

3. Microbial cell culture for plasmid vector propagation, purification and conjugation experiments.

4. Develop new targets for gene manipulation studies pertinent to her/his interests.

5. Up to 5 days per week is required on project responsibilities.


Incumbent will interact daily with supervisor for progress updates and experimental planning. Will also frequently interact with students and postdocs in the lab.


1) Education and Experience

Minimum of a M.S. degree in Marine Science
Minimum of one year of experience in a research laboratory setting
- 1 year experience with fluorescence microscopy analysis and cell isolation from field samples
-1 year experience with electroporation optimization and other transfection methods

2) Knowledge, Skills and Abilities required

a) Familiarity with basic and advanced techniques in molecular biology including, DNA, RNA isolation and purification, PCR, plasmid design and purification, Gibson Assembly for multi fragment reporter gene expression cassette design and construction microbial and phytoplankton cell culture.

b) The required skill set includes: sterile culture techniques, experience in phytoplankton and microbial culture and growth assays, phytoplankton isolation and species characterization from environmental samples, biological oceanographic field sampling experience, domoic acid quantification, biochemical and molecular biology skills including, PCR design and troubleshooting, plasmid preparations, gel electrophoresis, nucleic acid purification, Sanger sequencing, enzymatic assay optimization and general laboratory supply management.

c) Good communication skills including maintenance of accurate laboratory notebooks.

d) Competence in a range of software and platforms including MS Office, ImageJ, bioinformatics packages (eg. Geneious). R programming is a plus

e) Ability to work within a team framework and to self-direct development of independent research initiatives utilizing resources developed under this grant initiative.

3) Physical Requirements

Light lifting is required (up to 15 lbs.) for transport of culture media, movement of laboratory instrumentation, field sampling gear (net tows and other water samplers).

4) Complexity of Duties

Works fairly independently on assignments designed in conjunction with supervisor
SUPERVISORY RESPONSIBILITIES: None, though is responsible to guide, direct and train, as appropriate.

Job Category:  Non-Profit
Position type:  Full-Time
Salary:  Depends on Interview
Special Instructions:  APPLICATION PROCEDURE To apply for this position, you may submit a letter of interest and a resume or an application. An application and other information, including the job description, may be obtained from the Research Foundation through its web site at You may also obtain information in person by visiting the Research Foundation, located at 210 North 4th Street, 4th Floor, San Jose, CA (corner of St. James and North 4th Streets). A formal application for employment will be required of all candidates who are selected for a personal interview. Please address your letter of interest and resume or application directly to: San Jose State University Research Foundation Attn: HR/Job Code MLML RLT 210 North 4th Street San Jose, CA 95112 E-mail: Research Foundation employment is separate and distinct from San Jose State University or State of California employment. Research Foundation employees are not employees of either SJSU or of the state of California. The San Jose State University Research Foundation (SJSURF) is a non-profit auxiliary of San Jose State University. SJSURF is totally self supported. The majority of the organization’s funding comes from the federal government, and other public and private entities. With annual revenues totaling over $65 million, programs managed through SJSURF cover a rich diversity of applied research, public services, and educational related activities. SAN JOSE STATE UNIVERSITY RESEARCH FOUNDATION is an Equal Opportunity Employer and does not discriminate on the basis of race, color, creed, gender, religion, marital status, registered domestic partner status, age, national origin, ancestry, physical or mental disability, medical condition, sex, genetic information, sexual orientation, military and veteran status or any other consideration made unlawful by federal, state, or local laws. It also prohibits unlawful discrimination based on the perception that anyone has any of those characteristics, or is associated with a person who has or is perceived as having any of those characteristics. The SJSURF has implemented California State University Executive Order 1083 ( ). By policy, all SJSURF employees are mandated reporters for suspected child abuse or neglect and are expected to undergo formal training in accordance with the directives of the Executive Order. The SJSURF has also implemented California State University Policy Memorandum HR 2015 – 08 ( ). A background check (including a criminal records check) must be completed satisfactorily before any candidate can be offered a position with the SJSURF. Failure to satisfactorily complete the background check may affect the application status of applicants or continued employment of current SJSURF employees who apply for the position.
ref: (MLML RLT)
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